ea0004p83 | Steroids | SFE2002
Soory M
, Suchak A
The aim of this investigation is to establish the effect of alkaline phosphatase on androgen metabolism in human gingival fibroblasts (obtained local Ethical Committee approval) and its possible mediation by the alkaline phosphatase inhibitor levamisole and nicotine, using radiolabelled testosterone as substrate. Monolayer cultures of fibroblasts were established in Eagle's MEM. Duplicate incubations were performed in multiwell plates with 14C-testosterone as substrate, in the...